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VCU Massey Cancer Center

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Services and rates

Services provided

Flow cytometry analysis

  • Apoptosis assays
  • DNA and cell cycle analysis
  • Cell viability/cytotoxicity assays
  • Viable mitochondrial staining
  • Internal free Ca2+ measurement
  • Examination of cell surface antigens, internal antigens, and nuclear antigens
  • Multiplex cytokine/chemokine assay analysis
  • Phospho-flow cytometry
  • Cells ranging from bacterial cells to muscle cells can be analyzed
  • Multi-parameter analysis that extends up to 20-parameters, with 5-lasers
  • Image cytometry analysis that extends up to 6-parameters, with 4-lasers
  • Image cytometry for internalization, cellular compartment co-localization, or nuclear localization

High Speed Cell Sorting

  • Sorting of viable cell under sterile conditions
  • Single-cell sorting into 96-well plates
  • Sorting of cells extends up to 20-parameters, with 5 lasers

Data Analysis and Consultation

  • FlowJo software (most up to date version) available for annual license purchase or on-site usage for data analysis
  • FCSExpress v5 available for remote and on-site usage for data analysis
  • Data Analysis and figure preparation services
  • Troubleshooting and experimental design consultation
  • One-on-one training in data analysis or instrument operation

Rates

Flow analysis on LSRFortessaX-20

  • Instrument time - trained user per hour $54.00
  • Instrument time – facility per hour $99.00

Flow analysis on FACSCanto II

  • Instrument time - trained user per hour $46.00
  • Instrument time – facility per hour $93.00

Imaging flow analysis on ImageStream Mark II

  • Instrument time - trained user per hour $65.00
  • Instrument time – facility per hour $110.00

Flow sorting on FACSAria II or FACSAria Fusion

  • Instrument time - trained user per hour $65.00
  • Instrument time – facility per hour $110.00
  • Setup for sorts per hour - $45.00
  • Shutdown or cleanup for sorts per hour - $45.00

Surface plasmon resonance on Reichert SR7500DC SPR

  • Instrument time - trained user per hour $10.50
  • Instrument time – facility per hour $35.00

Flow/SPR supplies

  • Blue cap 35um nylon filter tube $1.10
  • Reichert Carboxymethyl Dextran chip $84.00 (High binding)
  • Reichert Carboxyl Surface Planar SAM chip $75.00 (Low binding)
  • Reichert Ni(NTA) chip $125.00
  • Reichert Strepavidin chip $125.00
  • SPR Reagents, each day $30.00

Data analysis/Training

  • Training (Instrument or Analysis) per hour $45.00
  • FlowJo License Purchase $345/year
  • Data Analysis – on site analysis software and data analysis computer usage per hour $5.00
  • Data Analysis – facility per hour $50.00

Other

  • Late arrival or No show per hour $45.00
  • Technician Time per hour $45.00

Consultation/Troubleshooting: Please inquire

External to VCU or Industry: Please inquire for pricing (Facility-run offerings only, instrument training is not available to external users)

Massey member subsidy benefit

Massey subsidizes 25 percent of the cost of members’ core laboratory usage for the following Massey-supported, fee-for-service shared resources:

  • Flow Cytometry Shared Resource
  • VCU Lipidomics/Metabolomics Shared Resource
  • Structural Biology Shared Resource
  • Tissue and Data Acquisition and Analysis Core
  • Transgenic/Knock-out Mouse Facility Shared Resource
  • Microscopy Shared Resource

To receive this member benefit, please initiate core lab services using a VCU index code associated with your name. When you are billed for core laboratory usage, the Rams FORCE system will automatically split the invoice between your index code and a Massey account established for this purpose. You will be charged 75 percent of the published rate and Massey will provide a 25 percent co-pay to cover the balance. Please direct any questions or concerns about the member subsidy benefit to Paul Fawcett, Ph.D. at pfawcett@vcu.edu or (804) 833-1959.

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