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Immune cells in the tonsil are identified using Akoya Bioscience’s 7-color OPAL staining. Total T-cell lymphocytes (CD3+), cytotoxic T-cells activated (CD3+, CD8+, granzyme B+), effector/memory cytotoxic T-cells (CD3+, CD8+, CD45+), regulatory T cells (CD3+, Foxp3+)-(CD3+, CD8+, Foxp3+). Green, CD3; red, CD8; light pink, CD45; magenta, Foxp3; orange, granzyme B; cyan, pan cytokeratin; blue, nucleus.
Jennifer Koblinski, PhD
Dr. Jennifer Koblinski, Assistant Professor in the Department of Pathology, continues to expand valuable services offered by the Cancer Mouse Models Core Laboratory (CMMCL). The CMMCL is now providing endoscopy for mice using the Coloview system (Karl Storz Veterinary Endoscopy). Biopsies can be collected during visual examination without sacrificing the animal allowing for more effective use of research models of chronic inflammatory bowel disease and colon carcinoma. Additionally, tumor cells can be injected orthotopically into the colon without surgery. A small animal hematology analyzer has also been purchased to provide complete blood counts. The CMMCL has continued to grow immunohistochemistry and immunofluorescent services, which includes immunofluorescent multiplexing up to nine colors using Akoya Bioscience’s OPAL staining. The CMMCL provides whole slide scanning for brightfield and fluorescent imaging with the Vectra Polaris (Akoya Bioscience), which provides automated quantitative pathology. The system integrates both multispectral imaging and automated slide scanning to better visualize, analyze, quantify and phenotype cell in tissue sections and tissue microarrays. Additionally, the CMMCL is continuing to developing patient-derived xenograft (PDX) models. It currently has four triple-negative breast cancer PDX developed from patients at VCU, and these are available for use.