PhD, Jagiellonian University, Poland (2003)
MSc, Jagiellonian University, Poland (1996)
My research is focused on understanding mechanism of compartmentalization of DNA replication, transcription, and repair in nuclei of mammalian cells. I study the relationship between dynamics of the respective nuclear compartments and processes contributing to development of cancer, such as motility of glioma cells. I participate as a collaborator in the development and testing of novel fluorescent probes for nuclei and other cell compartments. As the director of Massey's Microscopy Core Facility, I apply techniques developed in the course of my research to support users of the facility. I complement these methods with experience in operation and maintenance of imaging systems, including confocal, wide-field, two-photon and electron microscopes. I have successfully implemented several modalities of optical imaging of live and fixed biological material (cancer cells). These techniques include: time-lapse (fluorescence, backscattered light, DIC, phase contrast), multi-dimensional (confocal, multiphoton, deconvolution), time-resolved (fluorescence lifetime and correlation) and super-resolution (STED, STORM, SIM) microscopy. I combined microscopy-based morphological and functional studies of biological systems with flow cytometry, classic image cytometry and high-throughput screening for quantitative cancer studies. Working with these modalities included development and application of a wide range of techniques of image processing and statistical analysis.